MaxTaq DNA Polymerase is a modified and optimized thermostable enzyme blend containing Taq DNA Polymerase, Pfu DNA Polymerase and enhancing factors. It exhibits the 3' to 5' proofreading activity, resulting in considerably higher amplification fidelity than possible with unmodified Taq DNA Polymerase. Recommended for use in amplification to obtain DNA products up to 20kb.
1u is defined as the amount of enzyme that is required to catalyze the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. The reaction conditions are: 50mM Tris-HCl (pH 9.0 at 25°C), 50mM NaCl, 5mM MgCl2 , 200μM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [³H] dTTP ), 10μg activated calf thymus DNA and 100μg /ml BSA in a final volume of 50μl.
All preparations are assayed for contaminating endonuclease, exonuclease, and non-specific DNase activities. Functionally tested in DNA amplification.
20mM Tris-HCl (pH 8.0 at 22°C ), 100mM KCl, 0.5% Tween™ 20, 0.5% Nonidet- P40, 0.1mM EDTA, 1mM DTT and 50% glycerol. Store at -20°C.
|Catalog No||Description||Pack Size|
|PL2201||MaxTaq DNA Polymerase||200u, 5u/μl|
|PL2202||MaxTaq DNA Polymerase||500u, 5u/μl|
This Product Has Been Used In:
Yilmaz, S., Azizoglu, U., Ayvaz, A., Temizgul, Atciyurt, Z.B., Karaborklü, S. (2017) Cloning and expression of cry2Aa from native Bacillus thuringiensis strain SY49-1 and its insecticidal activity against Culex pipiens (Diptera: Culicidae)
Afrasiabi, A., et al. (2015) Analysis of Naturally Occuring Resistant Mutations to Hepatitis C Virus NS3 Protease Inhibitors A Preliminary Study in South of Iran. Jundishapur Journal of Microbiology.8(10).
Khodadad, M., et al. (2013) Constuction of Expressing Vectors Including Melanoma Differentiation-Associated Gene-7 (mda-7) Fused With the RGD Sequences for Better Tumor Targeting. Iranian Journal of Basic Medical Sciences. 18(8), p. 780-787.