AtTaq DNA Polymerase is a complex of specific anti-Taq monoclonal antibody with top quality thermostable Taq DNA Polymerase for automatic "Hot Start" amplification, resulting in greatly enhanced amplification specificity, sensitivity and yield. AtTaq DNA Polymerase catalyzes the polymerization of nucleotides into duplex DNA in the 5' to 3' direction with the presence of Mg²+ and has the 5' to 3' exonuclease activity.
1u is defined as the amount of enzyme that is required to catalyze the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. The reaction conditions are: 50mM Tris-HCl (pH 9.0 at 25°C), 50mM NaCl, 5mM MgCl2, 200μM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [³H]dTTP), 10μg activated calf thymus DNA and 100μg /ml BSA in a final volume of 50μl.
All preparations are assayed for contaminating endonuclease, exonuclease, and non-specific DNase activities. Functionally tested in DNA amplification.
20mM Tris-HCl (pH 8.0 at 22°C ), 100mM KCl, 0.5% Tween™ 20, 0.5% Nonidet- P40, 0.1mM EDTA, 1mM DTT, and 50% glycerol. Store at -20°C.
|Catalog No||Description||Pack Size|
|PL3201||AtTaq DNA Polymerase||200u, 5u/μl|