Pfu DNA Polymerase is an extremely thermostable proofreading DNA polymerase, suitable for applications requiring high temperatures synthesis of DNA. Pfu DNA Polymerase catalyzes the polymerization of nucleotides into duplex DNA in the 5' to 3' direction with the presence of Mg2+. It exhibits the 3' to 5' proofreading activity.
1u is defined as the amount of enzymes that is required to catalyze the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. The reaction conditions are: 50mM Tris-HCl (pH 9.0 at 25°C), 50mM NaCl, 5mM MgCl2 , 200μM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [³H]dTTP), 10μg activated calf thymus DNA and 100 μg/ml BSA in a final volume of 50 μl.
All preparations are assayed for contaminating endonuclease, exonuclease, and non-specific DNase activities. Functionally tested in DNA amplification.
50mM Tris-HCl (pH 8.0 at 22°C ), 100mM KCl, 0.1% Tween™ 20, 0.1% Nonidet- P40, 0.1mM EDTA, 1mM DTT, and 50% glycerol. Store at -20°C.
|Catalog No||Description||Pack Size|
|PL5201||Pfu DNA Polymerase||100u, 5u/μl|
|PL5202||Pfu DNA Polymerase||500u, 5u/μl|
This Product Has Been Used In:
Dastsooz, H., Dehghani, S.M., Imanieh, M.H., Haghighat, M., Moini, M., Fardaei, M. (2013) A new ATP7B gene mutation with severe condition in two unrelated Iranian families with Wilson disease. Gene. 514(1) Pp.48-53
Dastsooz, H. et al. (2013) DNA Sequence Fragment Containing C to A Mutation as a Convenient Mutation Standard for DHPLC Analysis Iran J Basic Med Sci.; 16(8): 946–949