Pfu DNA Polymerase (5u/ul)

Product Code: PL520x
Price: £39.00

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Description
Pfu DNA Polymerase is an extremely thermostable proofreading DNA polymerase, suitable for applications requiring high temperatures synthesis of DNA. Pfu DNA Polymerase catalyzes the polymerization of nucleotides into duplex DNA in the 5' to 3' direction with the presence of Mg2+. It exhibits the 3' to 5' proofreading activity.

Features

  • Ultra pure recombinant protein allows amplification up to 8kb.
  • 10X ViBuffer S provided for amplification of more than 5kb amplicon.

Unit Definition
1u is defined as the amount of enzymes that is required to catalyze the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. The reaction conditions are: 50mM Tris-HCl (pH 9.0 at 25°C), 50mM NaCl, 5mM MgCl2 , 200μM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [³H]dTTP), 10μg activated calf thymus DNA and 100 μg/ml BSA in a final volume of 50 μl.

Supplied With

  • 10X ViBuffer A (without MgCl2)
    500mM KCl, 100mM Tris-HCl (pH 9.1 at 20°C) and 0.1% Triton™ X-100. The buffer is optimized for use with 0.1-0.2mM of each dNTP.
  • 10X ViBuffer S
    160mM (NH4)2SO4, 500mM TrisHCl (pH 9.2 at 22°C), 17.5mM MgCl2 and 0.1% Triton™ X-100. The buffer is optimized for use with 0.35mM of each dNTP.
  • 50mM MgCl2.

Quality Control
All preparations are assayed for contaminating endonuclease, exonuclease, and non-specific DNase activities. Functionally tested in DNA amplification.

Storage Buffer
50mM Tris-HCl (pH 8.0 at 22°C ), 100mM KCl, 0.1% Tween™ 20, 0.1% Nonidet- P40, 0.1mM EDTA, 1mM DTT, and 50% glycerol. Store at -20°C.

Ordering Information

Catalog No Description Pack Size
PL5201 Pfu DNA Polymerase 100u, 5u/μl
PL5202 Pfu DNA Polymerase 500u, 5u/μl

Download Manual

Pfu DNA Polymerase

Publication
This Product Has Been Used In:

Dastsooz, H., Dehghani, S.M., Imanieh, M.H., Haghighat, M., Moini, M., Fardaei, M. (2013) A new ATP7B gene mutation with severe condition in two unrelated Iranian families with Wilson disease. Gene. 514(1) Pp.48-53

Dastsooz, H. et al. (2013) DNA Sequence Fragment Containing C to A Mutation as a Convenient Mutation Standard for DHPLC Analysis Iran J Basic Med Sci.; 16(8): 946–949

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