Viva 2-steps RT-PCR Kit (100 reactions)

Viva 2-steps RT-PCR Kit (100 reactions)
Product Code: RTPL12
Price: £179.00
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Description
Viva 2-steps RT-PCR kit is specially designed to provide reliable synthesis of full-length cDNA and convenient application of cDNA in PCR. M-MuLV Reverse Transcriptase synthesize complementary DNA strand initiating from a specific primer, oligo d(T) or random hexamer. The absence of RNase H enhances the synthesis of long cDNA as RNA strand does not degraded in DNA-RNA hybrid during first strand cDNA synthesis. With variety of kit options for standard PCR and long PCR, Viva 2-steps RT-PCR kit provides flexibility in an easy use format.

Features

  • Absence of RNase H activity allows high amount of full length cDNA synthesis with RNA templates up to 10kb.
  • Wide selection of primers, oligo d(T) or random hexamer. Taq DNA Polymerase and MaxTaq DNA Polymerase for amplification for short and long DNA fragments.

Kit Component

Viva cDNA Synthesis Kit

  • 10X M-MuLV RT Buffer, 250µl
  • 10,000u M-MuLV Reverse Transcriptase
  • 10mM dNTPs mix, 0.25ml
  • RNase-free water 3ml
  • 40µM Oligo d( T ), 100µl
  • 50ng/µl Random hexamer, 100µl

Viva PCR Kit

  • 500u Taq DNA Polymerase
  • 10X PCR Reaction Buffers
  • 50mM MgCl2
  • 10mM dNTPs Mix, 0.25ml

Storage

  • Store at -20°C

Ordering Information

Catalog No Description Pack Size
RTPL12 Viva 2-Steps RT-PCR kit with M-MuLV RT / Taq DNA Polymerase 100 applications
     
     
     

Download
Manual

Viva 2-Steps RT-PCR kit with M-MuLV RT / Taq DNA Polymerase

 

Publication
This Product Has Been Used In:

Bina, P., Kakhki, M.P., Sahraian, M.A., Behmanesh, M. (2017) The expression of lnc-IL-7R long non-coding RNA dramatically correlated with soluble and membrane-bound isoforms of IL-7Ra gene in multiple sclerosis patients

Jaroonwitchawan, T., Chaicharoenaudomrung, N., Namkaew, J., Noisa, P. 2017. Curcumin attenuates paraquat-induced cell death in human neuroblastoma cells through modulating oxidative stress and autophagy Neuroscience Letters. 636. Pp.40-47

Sheikhalizadeh, V., Hasani, A., Rezaee, M.A., Rahmati-yamchi, M., Hasani, A., Ghotaslou, R., Goli, H.R. (2017) Comprehensive study to investigate the role of various aminoglycoside resistance mechanisms in clinical isolates of Acinetobacter baumannii Journal of Infection and Chemotherapy. 23(2) pp74-79

Avci, E., Arıkoğlu, H., Kaya, D.E. (2016) Investigation of juglone effects on metastasis and angiogenesis in pancreatic cancer cells. Gene.588. Pp..74-78.

Babaei-Balderlou, F., & Khazali, H. (2016) Effects of Ghrelin on Sexual Behavior and Luteinzing Hormone Beta-subunit Gene Expression in Male Rats Journal of Reproduction Infertility2(1)17(2), p.88-96.

Jaroonwitchawan, T., Muangchang, P., Noisa, P. 2016. Inhibition of FGF signaling accelerates neural crest cell differentiation of human pluripotent stem cells. Biochemical and Biophysical Research Communications. 481(1-2). Pp.176-181

Nikoomanesh, F., et al (2016) Investigation of bcrI Gene Expression in Candida albicans Isolates by TR-PCR Technique and its Impact on Biofilm Formation JInfection Epidemiology and Medicine2(1), p. 22-24.

Pourhassanali, N., Roshan-Milani, S., Kheradmand, F., Motazakker, M., Bagheri, M.,Saboory, E. (2016) ) Zinc attenuates ethanol-induced Sertoli cell toxicity and apoptosis through caspase-3 mediated pathways. Reproductive Toxicology. 61. Pp97-103.

Abouhamzeh, B., et al. (2015) DNA Methylation and Histone Acetylation Patterns in Cultured Bovine Adipose Tissue-Derived Stem Cells (BADSCs). CELL Journal16(4), p. 466-475

Karimi, S., et al. (2015) Expression of Two Basic mRNA Biomarkers in Peripheral Blood of Patients with Non-Small Cell Lung Cancer Detected by Real-Time RT-PCR, Individually and Simultaneously Iranian Biomedical Journal. 19(1), p. 17-22.

Khodadad, N., et al. (2015) Prevalence of Influenza A(H1N1)pdm09 Virus Resistant to Oseltamivir in Shiraz, Iran, During 2012-2013 Jundishapur Journal of Microbiology8(8).

Madadgar,O., Nazaktabar, A., Keivanfar, H., Salehi, T.Z., Zadeh, S.L. (2015) Genotyping and determining the distribution of prevalent G and P types of group A bovine rotaviruses between 2010 and 2012 in Iran. 179(3-4) pp.190-196

Moghadam, F.H., et al. (2015) Differentiation of Rat Bone Marrow Mesenchymal Stem Cells into Adipocytes and Cardiomyocytes after Treatment with Platelet Lysate International Journal of Hematology-Oncology and Stem Cell Research.10(1), p. 21-29.

Tahoori, M.T., Pourfathollah, A.A., Soleimani, M., Vasheghani-Farahani, E., Mohammadzadeh, A., Amari, A., Hashemi, S.M., mossahebi-mohammadi, M. (2015) Fibroblasts feeder niche and Flt3 Ligand as a novel inducer of plasmacytoid dendritic cells development in vitro. International Immunopharmacology. 24. Pp.474-480.

Heidary, M., Rakhshi, N., Kakhki, M.P., Behmanesh, M., Sanati, M.H., Sanadgol, N., Kamaladinin, H., Nikravesh, A. (2014) The analysis of correlation between IL-1B gene expression and genotyping in multiple sclerosis patients.

Mostafavi, H., et al. (2014) Fluoxetin Upregulates Connexin 43 Expression in Astrocyte Basic and Clinical Neuroscience. 5(1), p. 74-79.

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