M-MuLV Reverse Transcriptase {RNase H¯}

M-MuLV Reverse Transcriptase {RNase H¯}
Product Code: ME2305
Price: £134.00
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Description
Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase is an RNA dependent DNA polymerase. It can synthesize a complementary DNA strand initiating from a primer using either RNA or single-stranded DNA as a template. The absence of RNase H activities enhances the synthesis of long cDNAs and therefore the enzyme is recommended for preparing long cDNAs.

Concentrations
100 - 500u/μl

Assay Conditions
50mM Tris- HCl (pH8.3), 6mM MgCl2, 10mM DTT, 0.4mM Poly(rA)•(dT)12-18, and 0.5mM [ 3H]dTTP in a reaction volume of 50μl./μl

Supplied With
10X Buffer M-MuLV RT

500mM Tris-HCl (pH 8.3 at 25˚C), 75mM KCl, 3mM MgCl2 and 10mM DTT. Store at -20˚C.

Storage Buffer
10mM K-Phosphate (pH 7.5), 0.1mM EDTA, 200mM NaCl, 7mM 2-mercaptoethanol and 50% glycerol. Store at -20˚C.

Thermal Inactivation
70˚C for 10 minutes

Unit Definition
1u is defined as the amount of enzyme that is required to incorporate 1nmol of dTTP into an acid-insoluble material in 10 minutes at 37˚C using Poly(rA)•oligo(dT).

Application

  • First strand cDNA synthesis.
  • DNA labeling.
  • RNA analysis by primer extension.

Quality Control
Purified free of contaminating endonucleases and exonucleases.

Ordering Information

Catalog No Description Pack Size
ME2305 M-MuLV Reverse Transcriptase (RNase H¯) 10000u

Download Manual

M-MuLV Reverse Transcriptase (RNase H¯)

Publication
This Product Has Been Used In:

Kaplan, S. et al. (2016) The Potential of Microarray Databases to Identify Tissue Specific Genes Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 22(1), p. 29-35.

Tahoori, M.T., Pourfathollah, A.A., Soleimani, M., Vasheghani-Farahani, E., Mohammadzadeh, A., Amari, A., Hashemi, S.M., mossahebi-mohammadi, M. (2015) Fibroblasts feeder niche and Flt3 Ligand as a novel inducer of plasmacytoid dendritic cells development in vitro. International Immunopharmacology. 24. Pp.474-480.

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