The GF-1 AmbiClean Kit (Gel & PCR) is designed for rapid DNA recovery from agarose gel and PCR clean-up of DNA bands ranging from 100bp to 20kb. Special buffer provide the correct salt concentration and pH for efficient recovery (80-90%) of DNA from both PCR product and agarose gel from TAE or TBE buffers. The kit is well suited for the removal of agarose, excess dNTPs, short oligo fragments, mineral oil, enzymes from a PCR reaction product, proteins after restriction enzyme treatment and dephosphorylation, residual dye and ethidium bromide. This kit also allows for concentration of DNA, changing of buffers and desalting.
- 90% of recovery achievable
- Purification process less than 15 minutes
- High pure DNA ready-to-use for routine molecular biology applications such as restriction enzyme digestion, PCR, ligation, DNA sequencing, probe preparations, etc.
- Buffer DB
- Wash Buffer (concentrate)
- Elution Buffer
|Catalog No||Description||Pack Size|
|GF-GC-050||GF-1 AmbiClean Kit (Gel & PCR)||50 preps|
|GF-GC-100||GF-1 AmbiClean Kit (Gel & PCR)||100 preps|
|GF-GC-200||GF-1 AmbiClean Kit (Gel & PCR)||200 preps|
This Product Has Been Used In:
Said, M.B., Belkahia, H., Mabrouk,N.E., Saidani, M., Hassen, M.B., Alberti, A., Zobba, R., Bouattour, S., Bouattour, A., Messadi, L. (2017) Molecular typing and diagnosis of Anaplasma spp. closely related to Anaplasma phagocytophilum in ruminants from Tunisia. Ticks and Tick-borne Diseases.8. Pp..412-422
Said, M.B., Belhakia, H., Mabrouk, N.E., Saidani, M., Alberti ,A., Zobba,R., Cherif, A., Mahjoub, T., Bouattour, A., Messadi, L. (2017) Anaplasma platys-like strains in ruminants from Tunisia. Infection, Genetics and Evolution 49. Pp..226-233.
Azwai, S.M. et al. (2016) Isolation and Molecular Identification of Vibrio spp. By sequencing of 16S rDNA from seafood, meat and meat products in Libya Open Veterinary Journal, 6(1), p. 36-43.
Hamidinejat, H., et al. (2015) Development of an Indirect ELIS using Different Fragments of Recombinant Ncgra 7 for Detection of Neospora caninum Infection in Cattle and Water Buffalo. OIran Journal of Parasitology. 10(1), p.69-77.
Khalafalla, A.I., Al-Busada, K.A., & El-Sabagh, I.M. (2015) Multiplex PCR for Rapid Diagnosis and Differentiation of Pox and Pox-like Diseases in Dromedary Camels. Virology Journal. 12(102), p. 1-10.
Nasanit, R., et al (2015) Assesment of Epiphytic Yeast Diversity in Rice (Oryza sativa) Phyllospehere in Thailand by a Culture-independent Approach Antonie van Leeuwenhoek.Springer. 107(6), p.1475-1490
Belkahia, H., Said, M.B., Hamdi, S.E., Yahiaoui, M., Gharbi, M., Daaloul-Jedidi, M., Mhadbi, M., Jedidi, M., Darghout, M.A., Klabi, I., Zribi, L., Messadi, L. (2014) ) First molecular identification and genetic characterization ofAnaplasma ovis in sheep from Tunisia. Small Ruminant Research. 121. Pp.404-410.